Title: Inhibitory effects of Ciwujianoside B on apoptosis induced by hydrogen peroxide in neonatal rat cardiomyocytes
Abstract:Objective To observe the effect of Ciwujianoside B(C-B) on apoptosis induced by hydrogen peroxide (H2O2) in cardiomyocytes and the expressions of its related regulating proteins,and clanify the inhibi...Objective To observe the effect of Ciwujianoside B(C-B) on apoptosis induced by hydrogen peroxide (H2O2) in cardiomyocytes and the expressions of its related regulating proteins,and clanify the inhibitory effect of C-B on apoptosis induced by injury of oxidative stress in cardiomyocytes.Methods The neonatal rat cardiomyocytes were primary cultivated and the cells which beat autonomously well were randomly divided into normal control group,H2O2 group,100 mg·L-1 C-B group and 200 mg·L-1 C-B group.The cardiomyocytes were exposed to 200 μmol·L-1H2O2 for 120 min to induce apoptosis.The morphological changes of cardiomyocytes were observed by microscope,the survival rate was detected by MTT.The apoptotic rate was analyzed by flow cytometry labelled with AnnexinⅤ-FITC.The activity of Caspase-3 was detected by spectrophotometric method.The expressions of Caspase-3,PARP,Bcl-2 and Bax proteins were determined by immunoblotting.Results Compared with normal control group,the injuried cardiomyocytes were seen and the survival rate was decreased significantly (P0.001),the apoptotic rate and Caspase-3 activity were increased (P0.001 or P0.01);the expressions of Caspase-3,Bax and the degradation of PARP were raised,and the expression of Bcl-2 was decreased in 200 μmol·L-1H2O2 group.Compared with 200 μmol·L-1H2O2 group,the injuried changes of cardiomyocytes were reduced,the survival rates were significantly increased (P0.05 or P0.01),the apoptotic rates were decreased (P0.001);the activities of Caspase-3 were decreased significantly (P0.05 or P0.01);the expressions of Caspase-3 and Bax were decreased,the degradation of PARP was attenuated,and the expressions of Bcl-2 were increased in 100 and 200 mg·L-1 C-B groups.Conclusion C-B can obviously inhibit the apoptosis induced by H2O2 in cardiomyocytes,its mechanism may be related to the regulation of the expressions of the apoptosis-related proteins Caspase-3,Bax and Bcl-2.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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