Title: Salvianolate protects rat cardiomyocytes from high-glucose-induced apoptosis
Abstract:Objective To investigate the effects of salvianolate on high glucose- induced apoptosis of rat cardiomyocytes( H9C2 cells) and the underlying mechanisms. Methods Rat myocardial cell line H9C2 were div...Objective To investigate the effects of salvianolate on high glucose- induced apoptosis of rat cardiomyocytes( H9C2 cells) and the underlying mechanisms. Methods Rat myocardial cell line H9C2 were divided into control group,high- glucose group and high- glucose plus salvianolate( 50 mg /L,150 mg /L and 450 mg /L) groups. The cell apoptosis was examined by flow cytometry,while the mRNA and protein expression of Bcl- 2 and Caspase- 3 were examined by real time RT- PCR and Western blot,respectively. Results The apoptosis rate in high- glucose group was significantly increased than that in control group( P 0. 05). Compared with high glucose group,the apoptosis rate was significantly reduced in salvianolate groups with significant down- regulation of Caspase- 3 and up- regulation of Bcl-2( P 0. 05). Although salvianolate provided protective effect on H9C2 cells against high- glucose- induced apoptosis, no significant difference was observed between the high concentration and low concentration groups( P 0. 05). Conclusion Salvianolate can affect the expression of Bcl- 2 and Caspase- 3,and ultimately inhibits the high- glucose- induced apoptosis of rat cardiomyocytes.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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