Title: Inhibition of probucol on cultured neonatal rat cardiomyocyte apoptosis induced by hydrogen peroxide
Abstract:Aim:To observe the effect of probucol on cardiomyocyte apoptosis induced by hydrogen peroxide(H 2 O2) and to explore its possible mechanisms.Methods:Cultured neonatal rat cardiomyocytes were randomly ...Aim:To observe the effect of probucol on cardiomyocyte apoptosis induced by hydrogen peroxide(H 2 O2) and to explore its possible mechanisms.Methods:Cultured neonatal rat cardiomyocytes were randomly divided into 4 groups,and were given 0,1,10,and 100μmol/L probucol pretreatment for 6 h,respectively,and then induced by 0.2 mmol/L H2 O2 for 6 h.Apoptosis cells were assessed by flow cytometry.The mRNA expression of Bcl-2 and Bax in cardiomyocytes was determined by RT-PCR.The superoxide dismutase(SOD)activity and malondialdehyde(MDA)contents in cells were tested by xanthine oxidase method and color reaction of thiobarbituric acid method,respectively.Results:Probucol decreased cardiomyocyte apoptosis induced by H2 O2 and Bax mRNA expression,increased Bcl-2 mRNA expression,increased SOD activity and decreased the MDA content significantly.All these effects were in a dose-dependent manner.Conclusion:Probucol could inhibit cardiomyocyte apoptosis induced by H 2 O2 partly by reducing the oxidative stress level.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot