Title: HPLC-ELSD Fingerprint of carbohydrate constituents in Morinda officinalis from different habitats
Abstract:Objective To establish HPLC fingerprint analysis method of carbohydrate constituents in Morinda officinalis collected from different habitats.Methods HPLC Method was employed and a Columns Inertsil NH...Objective To establish HPLC fingerprint analysis method of carbohydrate constituents in Morinda officinalis collected from different habitats.Methods HPLC Method was employed and a Columns Inertsil NH2(250 mm×4.6 mm,5 μm)with acetonitrile-water as mobile phase by gradient elution was used,the flow rate was 1.2 mL/min,column temperature was room temperature.Detector was ELSD and the temperature of drift tube was 100 ℃.The flow rate of N2 was 2.2 L/min,Helium degasification on-line with flow rate was 20 mL/min.The fingerprints evaluating software by computer-assisted for the similarity was used to process the experiment data and compare the similarity of HPLC fingerprint of M.officinalis from different habitats.Results Except for two batches,the fingerprint similarity value of other eight batches of herbs was above 0.94 compared with system generation spectrum and 13 common characteristic peaks were demonstrated.The first and second peaks were D-fructose and sucrose,respectively compared with the retention time of standard sample.The results showed that the carbohydrate constitutes of M.officinalis were similar among herbs from different habitats,but their relative ratios are significantly different.Conclusion The method in this experiment is stable and reproducible,which could distinguish the internal quality and provide the reference for quality control of M.officinalis.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot