Title: Fingerprint analysis of Hypericum performatum L. by HPLC
Abstract:Objective To establish a method of fingerprint analysis on Hypericum performatum L. by HPLC. Methods The HPLC method was used,and chromatographic conditions were as follow:DiamonsilC18column(250 mm ×...Objective To establish a method of fingerprint analysis on Hypericum performatum L. by HPLC. Methods The HPLC method was used,and chromatographic conditions were as follow:DiamonsilC18column(250 mm × 4.6 mm,5 μm);acetonitrile(contain 10% methyl alcohol and 0. 2% methanoic acid)- water( contain20% methyl alcohol and 0. 2% methanoic acid) system as mobile phase at flow rate 1. 0 mL /min; detective wavelength at 270nm;column temperature was room temperature. Results The fingerprint of Hypericum performatum L. was established. 13 common peaks were found in the HPLC fingerprint of Hypericum performatum L.. Among 14 batches of samples,good similarities with correlation coefficients over 0. 9 were found in the fingerprints between the 13 batches of samples from different sources and standard fingerprint,which could be utilized for the identification of Hypericum performatum L.. Conclusion The method is accuate,simple and good reproducibility and laid the foundation for the quality standard of Hypericum performatum L..Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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