Abstract:Objective: To establish HPLC fingerprint of Morus alba,which was expected to be standards of quality control and identification of the Chinese crude drug.Method: The HPLC method was used,chromatograph...Objective: To establish HPLC fingerprint of Morus alba,which was expected to be standards of quality control and identification of the Chinese crude drug.Method: The HPLC method was used,chromatography conditions were Agilent Eclipse XDB-C18(4.6 mm × 250 mm,5 μm) column with gradient mobile phase of acetonitrile-0.1% acetic acid,UV detection wavelength was 290 nm and the column temperature was 30 ℃ with the flow rate of 1.0 mL.min-1,the sample injection was 10 μL.Result: HPLC fingerprints of 13 samples of M.alba were established.16 mutual peaks were selected as the fingerprint peaks in 11 samples.Among the obtained fingerprints,most of the detected peaks were separated effectively.11 samples had high similarities.Conclusion: The established HPLC fingerprint has desirable accuracy,repeatability and stability,which can be used for one of the quality control of M.alba.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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