Title: Study on HPLC Fingerprint of Galla Chinensis
Abstract:OBJECTIVE: To establish the HPLC fingerprint of Galla Chinensis. METHODS: The separation was performed on Kromasil C18(250 mm×4.6 mm, 5 μm) column, with mobile phase consisted of 1% glacial acetic aci...OBJECTIVE: To establish the HPLC fingerprint of Galla Chinensis. METHODS: The separation was performed on Kromasil C18(250 mm×4.6 mm, 5 μm) column, with mobile phase consisted of 1% glacial acetic acid-methanol (gradient elution) at the flow rate of 1.0 ml/min. The detection wavelength was set 280 nm, and column temperature was 25 ℃. The systematic cluster analysis of 14 batches of samples was conducted with SPSS13.0 software; the similarity of them was evaluated using fingerprint similarity evaluation system. RESULTS: 14 common peaks were identified using gallic acid as internal reference peak; among 14 batches, the similarity of 12 batches were more than 0.900 as recommended products; other 2 batches were lower than 0.900 as non-recommended products. It was same to the results of cluster analysis. CONCLUSIONS: Established method is sensitive, reproducible and specific, and can be used for the quality evaluation for Galla Chinensis.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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