Title: Study on specific chromatogram of rosmarinus officinalis by HPCE
Abstract:Objective:To establish the specific chromatogram of water-soluble constituents of rosmarinus officinalis by capillary electrophoresis.Methods:Twenty mmol·L-1 borax(pH=9.50)was used as background elect...Objective:To establish the specific chromatogram of water-soluble constituents of rosmarinus officinalis by capillary electrophoresis.Methods:Twenty mmol·L-1 borax(pH=9.50)was used as background electrolyte,and operation voltage of 12 kV was applied.UV-detection was adopted at the wavelength of 300 nm.Samples were introduced at a height of 10 cm for 5 s.Result:Samples of rosmarinus officinalis from 10 different origins were selected for analysis.Based on the value of rate of common electrophoretic peaks fi≥90%,a set of electrophoretic fingerprint atlas of 11 peaks was determined for flavones in rosmarinus officinalis samples from 10 different areas.Good similarities between the electrophoretic specific chromatogram of the samples and the standards were obtained.Relative standard deviation among the values of relative migration times of various specific peaks obtained at different time points of sample introduction was less than 5.0%,and values of RSD's for the relative peak areas were in the range from 2.7% to 8.0%.The results obtained proved that the sample was stable within 36 h.Conclusion:The method is simple and accurate,which could be an effective method for the quality control of rosmarinus officinalis.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 1
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