Abstract:Objective:To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Hibiscus Mutabilis.Method:HPLC method was set up by Waters Xbridge C18column(4....Objective:To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Hibiscus Mutabilis.Method:HPLC method was set up by Waters Xbridge C18column(4.6 mm × 250 mm,5 μm) with gradient elution;the mobile phase was comprised of acetonitrile-1% acetic acid solution,the UV detection wavelength was at 370 nm with a flow rate of 1.0 mL·min-1,and column temperature was at 25 ℃.Result:There were 10 common peaks in the fingerprints of H.mutabilis based on 10 habitats.Sum of peak areas was larger than 90% of total area.As compared with standard sample,rutin,hyperin and quercitrin were checked out.Conclusion:The method is reliable,accurate and can be used as a quality control method for H.mutabilis.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot