Title: Study on Fingerprints of Woodwardiae unigemmmata from Different Habitats by HPLC
Abstract:Objective: To establish the fingerprints of Woodwardiae unigemmmata from different habitats by HPLC.Method: HPLC was applied to detect W.unigemmmata samples from 9 different habitats with a DiamonsilT...Objective: To establish the fingerprints of Woodwardiae unigemmmata from different habitats by HPLC.Method: HPLC was applied to detect W.unigemmmata samples from 9 different habitats with a DiamonsilTMC18 column by acetonitrile A-water B as mobile phase(gradient elute: 0-60 min,22%-100%A).The flow rate was 1.0 mL·min-1.The column temperature was at 25 ℃ and the UV detection wavelength was set at 265 nm.Result: The HPLC fingerprints of W.unigemmmata from different habitats were established and the correlation coefficients of them were calculated.Furthermore,two peaks in HPLC chromatogram were identified as kaempferol-3-O-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside,kaempferol-3-O-α-L-(4-O-acetyl)-rhamnopyranoside-7-O-α-L-rhamnopyran-oside respectively.Conclusion: The method is simple,reproducible and can be used for quality control of W.unigemmmata.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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