Title: Protective Effect of Acetaminophen on Cultured Cardiac Myocytes Jnjured by H_2O_2
Abstract:Objective The purpose of this investigation was to determine the role of acetaminophen on cultured neonatal rat cardiac myocytes injured by H2O2.Methods Cultured neonatal rat cardiac myocytes were pre...Objective The purpose of this investigation was to determine the role of acetaminophen on cultured neonatal rat cardiac myocytes injured by H2O2.Methods Cultured neonatal rat cardiac myocytes were pretreated with acetaminophen(20 μmol·L-1,60 μmol·L-1 and 180 μmol·L-1) for 1 h before treated with H2O2 (0.2 mmol·L-1) for 6 h. The activity of dehydrogenase in mitochondria was detected by MTT assay. The activity of LDH, content of MDA, activity of SOD and caspase-3 were determined by LDH, MDA, SOD and caspase-3 kit.Results The descent of the activity of dehydrogenase in mitochondria detected by (MTT), the increase of LDH in culture medium, the elevation of MDA and the increase activity of caspase-3 induced by treatment of H2O2 were significantly reversed by acetaminophen. The effect of acetaminophen was increased with the rising of its concentration.Conclusions Acetaminophen could decrease the injury of cardiomyocytes induced by H2O2, inhibit the apoptosis of cardiomyocytes induced by H2O2, and this cardioprotective effect may be related to the antioxidant nature of the drug.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot