Title: Study on the mechanisms of rat cardiomyocyte injury induced by exogenous spermine
Abstract:Objective To study the possible mechanisms of cardiomyocyte injury induced by exogenous spermine(Sp).Methods Cardiomyocytes from 2~3 day-old rats were cultured in the RPMI-1640 medium.The morphologica...Objective To study the possible mechanisms of cardiomyocyte injury induced by exogenous spermine(Sp).Methods Cardiomyocytes from 2~3 day-old rats were cultured in the RPMI-1640 medium.The morphological changes of cardiomyocytes were observed using microscope and transmission electron microscope.The cardiomyocyte viability was detected by MTT assay,and the lactate dehydrogenase(LDH) activity in the medium was measured.Results Sp(100μmol/L) induced the injury of cultured neonate rats cardiomyocytes.The LDH leakage increased,the cardiomyocyte viability decreased significantly,and the ultrastructure of cardiomyocytes was injuried severely in the spermine-treated group.After pretreatment with VP,SOD,ATP and DEX in certain concentration,the LDH activity was decreased,and the cardiomyocyte viability was higher,and the ultrastructural injuries were improved compared with Sp-treated group.Conclusion Exogenous spermine could induce the injury of cultured neonatal rat cardiomyocytes,and its mechanisms may relate to intracellular calcium overload,free radical increase,energy disorder,and inflammatory mediators release.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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