Title: Effects of adiponectin against hydrogen peroxide induced myocardial injury in cultured rat neonatal cardiomyocytes
Abstract:AIM:To observe the effects of adiponectin on injury of the 3-4 d SD rat cardiomyocytes induced by the intervention of H2O2.METHODS:Primary cardiomyocytes were obtained from neonatal rat and were cultu...AIM:To observe the effects of adiponectin on injury of the 3-4 d SD rat cardiomyocytes induced by the intervention of H2O2.METHODS:Primary cardiomyocytes were obtained from neonatal rat and were cultured by enzymatic digestion methods.The molecular marker was observed by α-actin immunocytochemistry.Primary cultured 3-4 d cells were used in experiment,and the injury model was established by H2O2,and adiponectin and Ara-A were used for pre-treatment before cell culture.The morphological change of cardiomyocytes was observed under electron microscope.The contents of LDH,MDA and the activity of SOD were measured.The apoptosis of cardiomyocytes was detected by agarose gel electrophoresis and Annexin V/PI staining with flow cytometry.RESULTS:Adiponectin pretreatment significantly decreased the release of LDH(P0.05)and the content of MDA(P0.05),and increased the activity of SOD(P0.05) as compared to H2O2 injury group.The brightness of DNA ladder attenuated and the rate of apoptosis also decreased(P0.05) compared to H2O2 injury group.Ara-A(1 mmol/L) blocked the protection of adiponectin partly.CONCLUSION:H2O2 induces the injury and apoptosis of cardiomyocytes.The protective effect of adiponectin is through AMPK pathway.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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