Title: Protection of Compound Sanqi Chuanxiong Soft Capsule (KXSC) on Primary Culture Cardiac Myocyte Injured by Hydrogen Peroxide
Abstract: To establish a method for primary culture of cardiac myocyte injured by H2O2 and to investigate the cardio-protective effect of KXSC. After adding KXSC, the primary culture cardiac myocyte was injured by 0. 1 mmol/L H2O2. The activity of lactic dehydrogenase (LDH), SOD and mitochondrion succinic dehydrogenase (MSDH) and the content of MDA were assayed. The cardiac myocyte apoptosis was observed by flow cytometry and electrophoresis. KXSC significantly decreased the activity of LDH and the content of MDA in culture supernatant, increased the activity of SOD and MSDH , and decreased the percentage of apoptosis of cardiac myocyte treated with H2O2. KXSC has the protective effects on cardiomyocytes injured by H2O2 through improving cell antioxidant ability.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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