Title: Determination of Vitexin Rhamnoside in Hawthorn Leaves Extracts
Abstract:Objective:To establish a accurate sensitive and reproducible method for the determination of vitexin rhamnoside in Hawthorn leaves extracts.Methods:The column was Zorbax SB-C18(250mm×4.6 mm,5 μm),The ...Objective:To establish a accurate sensitive and reproducible method for the determination of vitexin rhamnoside in Hawthorn leaves extracts.Methods:The column was Zorbax SB-C18(250mm×4.6 mm,5 μm),The mobile phase was composed of A:acetonitrile,B:water;gradient elution profile was 0~5 min,A:5%~10%,B:95%~90%,5~10 min,A:10%~18%,B:90%~82%,10~30 min,A: 18%,B: 82%,with a flow rate of 1.0 mL/min,detection wave length was 334 nm and the column temperature was 30℃.Results:The 1inear range of vitexin rhamnoside was 4.296~64.440 μg/mL(r=1);the average recovery was 99.19%(RSD=1.94%).Conclusion:The method is simple,accurate and sensitive for the determination of vitexin rhamnoside in Hawthorn leaves extracts.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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