Title: Determination of Vitexin and Apigenin-7-O-β-D-Pyranglycu Ronide in Broussonetia papyrifera by HPLC
Abstract:[Objective]To determine the content of vitexin and apigenin-7-O-β-D-pyranglycuronide in Broussonetia papyrifera leaves.[Method] Using absolute methanol-1% acetic acid glacial(30∶70,V/V) as mobile phas...[Objective]To determine the content of vitexin and apigenin-7-O-β-D-pyranglycuronide in Broussonetia papyrifera leaves.[Method] Using absolute methanol-1% acetic acid glacial(30∶70,V/V) as mobile phase,the content of vitexin in B.papyrifera leaves was measured by HPLC method at the detection wavelength of 359 nm.Using absolute methanol-0.05% phosphoric acid(40∶60,V/V) as mobile phase,the content of apigenin-7-O-β-D-pyranglycuronide was measured by HPLC method at the detection wavelength of 324 nm.[Result] There was a good linearity in the concentration range of 0.056-0.488 μg/ml for vitexin(R=0.999 2).The average recovery of vitexin was 99.2% with the RSD of 1.42%.Apigenin-7-O-β-D-pyranglycuronide presented a good linearity in the concentration range of 1.808-4.068 μg/ml(R=0.999 8).The average recovery of apigenin-7-O-β-D-pyranglycuronide was 100.9% with the RSD of 1.58%.[Conclusion]This method that takes advantages of simplicity and good separating effect could be used for establishing the quality standard of B.papyrifera leaves.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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