Title: Simultaneous Determination of Vitexin and Isovitexin in Leaves of Santalum Album L.by HPLC
Abstract:Objective To develop a HPLC method for simultaneous determination of vitexin and isovitexin in leaves of Santalum album L.Methods The separation was achieved on a C18column(4.6 mm × 250 mm,5 μm.Agilen...Objective To develop a HPLC method for simultaneous determination of vitexin and isovitexin in leaves of Santalum album L.Methods The separation was achieved on a C18column(4.6 mm × 250 mm,5 μm.Agilent,USA) with a mobile phase consisting of methanol-0.5 % acetic acid(40 ∶ 60).The flow rate was 1 mL.min-1.The column temperature was maintained at 30 ℃.The detection wavelength was at 338 nm.Results The linear range of vitexin was 7.625~488.0 μg.mL-1.The recoveries of high-,middle-and low-concentration of vitexin were 96.38 %,97.23 % and 97.96 % respectively,and the RSD was 1.29 %~1.83 %.The linear range of isovitexin was 8.047~515.0 μg.mL-1.The recoveries of high-,middle-and low-concentration of isovitexin were 97.81 %,96.13 % and 97.98 % respectively,and the RSD was 1.31 %~1.81 %.The average content of three batches of samples was 1.13 %~1.25 % for vitexin and 1.20 %~1.46 % for isovitexin.Conclusion The method is accurate and repeatable,which can be used for quality evaluation of vitexin and isovitexin of leaves of Santalum album L.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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