Title: Simultaneous Determination of Vitexin and Isovitexin in Microcos paniculata by RP-HPLC
Abstract:OBJECTIVE:To develop a method for simultaneous determination of vitexin and isovitexin in Microcos paniculata.METHODS:RP-HPLC method was adopted.The determination was performed on SHISEIDO Capcell pak...OBJECTIVE:To develop a method for simultaneous determination of vitexin and isovitexin in Microcos paniculata.METHODS:RP-HPLC method was adopted.The determination was performed on SHISEIDO Capcell pak MG C 18(250 mm×4.6 mm,5 μm)with mobile phase composed of methanol-0.4% formic acid(30:70,V/V)at the flow rate of 1.0 ml/min.The detection wavelength was set at 339 nm and the column temperature was maintained at 30 ℃.RESULTS:The linear ranges of vitexin and isovitexin were 53.40-1 334.00 ng(r=0.999 9)and 73.90-1 847.00 ng(r=0.999 9).The average recoveries were 99.00%(RSD= 1.87%,n=9)and 99.37%(RSD=1.77%,n=9).CONCLUSION:The method is reproducible,simple and accurate,and it provides reference for quality evaluation and resource application of M.paniculata.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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