Title: Construction of Detection Method for Water-soluble Isoflavone Content in Soybean Milk
Abstract:The method for the detection of isoflavone in soybean milks by the High Performance Liquid Chromatography( HPLC) was optimized. And ultra high-speed centrifugation was used for the determination of wa...The method for the detection of isoflavone in soybean milks by the High Performance Liquid Chromatography( HPLC) was optimized. And ultra high-speed centrifugation was used for the determination of water-soluble isoflavone contents including Daidzin,Genistin,Daidein and Genistein. The result showed that under the conditions of a C18 column( 150 mm ×4. 6 mm,5. 0 μm),mobile phase A containing 0. 1%( V /V) acetic acid in ultrapure aqueous,mobile phase B containing0. 1%( V /V) acetic acid in methanol,a gradient mobile phase at 260 nm,a flow rate of 0. 8 mL·min- 1,the four different kinds of isoflavones in soybeanmilk could be well separated and detected which good linearities with the correlation coefficient of daidzin,genistin,daidein,genistein were 0. 999 2,0. 999 1,0. 999 3 and 0. 9994,respectively. The result also showed that there were significant differences among the four kinds of isoflavone contents in different types of soymilk product. Furthermore,homemade soymilk has a significantly higher isoflavone content than common commercially soymilk. The method constructed can be used for the fast and accurate detection of isoflavone in different kinds of soybean milk.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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