Title: Determination of Daidzein and Genistin in Soybean Isoflavone Flour by HPLC
Abstract:OBJECTIVE To establish HPLC method for the determination of Daidzein and Genistin in soybean.METHODS C18 column,The mobile phase was composed of methanol-water-acetic acid(40∶60∶1.2)with detection wav...OBJECTIVE To establish HPLC method for the determination of Daidzein and Genistin in soybean.METHODS C18 column,The mobile phase was composed of methanol-water-acetic acid(40∶60∶1.2)with detection wavelength at 254 nm. RESULTS It is a good linear relation of the linear range and the concentration of Daidzein within 0.008~0.4 μg and the concentration of Genistin within 0.0068~0.34 μg.CONCLUSION This method was convenient,rapid and reproducible,and suitable for the quality control of the determination of Daidzein and Genistin in soybean.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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