Title: Determination of isoflavones in soybean product by HPLC
Abstract:Two isoflavones in soybean product,genistein and daidzein,were determined by High performance liquid chromatography(HPLC).The soybean product was degreased by n-hexane at above 90℃ for 10 h,then extra...Two isoflavones in soybean product,genistein and daidzein,were determined by High performance liquid chromatography(HPLC).The soybean product was degreased by n-hexane at above 90℃ for 10 h,then extracted with 80% methanol at 80℃.Chromatographic separation conditions were: BDS Hypersil C18 column(250×4.6×5 mm),detection wavelength at 260 nm,column temperature at 35℃,gradient elution with a 1.0 ml/min flow rate,and a mobile phase containing methanol:(0.5%) acetic acid solution.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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