Title: Determination of Genistein in Soybean Isoflavone Sustained-release Pellet Capsules by RP-HPLC
Abstract:In order to validly control the quality of soybean isoflavone sustained-release pellet capsules,a method for determination of genistein in it was established using the method of RP-HPLC for determinat...In order to validly control the quality of soybean isoflavone sustained-release pellet capsules,a method for determination of genistein in it was established using the method of RP-HPLC for determination.Chromatographic conditions include:column:ZOBAX SB-C18(5 μm,250 mm×4.6 mm);mobile phase:acetonitrile-0.1% H3PO4 solution(40:60);detection:UV 262 nm;temperature:35℃;flow rate:1.0 mL·min-1;sample size:5 μL.Using one point external standard method to quantitate.Genistein had a good linear relationship in the range of 20.88-208.2 ng,regression equation was Y=4.38X-2.69,R=0.999(n=6).The average recovery of genistein was 99.97%,RSD=0.87%(n=9).The method is rapid,accurate,reliable and can be used to determine genistein in the soybean isoflavone sustained-release pellet capsules.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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