Title: Effect of microRNA-3175 on growth and invasion of glioma
Abstract:Objective
To investigate the expression of microRNA (miR) -3175 in glioma and its effect on the growth and invasion of glioma.
Methods
The expression levels of miR-3175 in 40 human glioma tissu...Objective
To investigate the expression of microRNA (miR) -3175 in glioma and its effect on the growth and invasion of glioma.
Methods
The expression levels of miR-3175 in 40 human glioma tissues and glioma cell A172 and U87 were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). After glioma cells transfection of miR-3175 inhibitor with Lipofectamine 2000, qRT-PCR detected the degree of inhibition of miR-3175. The effects of miR-3175 on glioma cell proliferation, cell cycle, apoptosis, and invasion were detected by MTT assay, flow cytometry assay and Transwell assay, respectively. Western blot was used to analyze the relationship between miR-3175 and apoptosis-related protein p53, Bcl-2, cytochrome C, and Caspase-3.
Results
The expression level of miR-3175 in glioma was increased obviously (compared with the control group P<0.05). The expression level of miR-3175 was decreased significantly after transfection of miR-3175 inhibitors (P<0.05). The miR-3175 inhibitors could effectively inhibit the proliferation and invasion abilities of glioma cells. The proliferation ability of U87 and A172 decreased 23.56±8.06% and 40.33±12.94% respectively; the number of the invasive cells reduced 47.83±4.33% and 45.13±5.03% respectively; at the same time, the MiR-3175 inhibitors could obviously increase the ratio and apoptosis rate of glioma cell G0/G1 phase, the ratio of A172 and U87 cell G0/G1 phase increased 13.51% and 13.22% respectively; the apoptosis rate increased 8.85±2.51% and 12.31±5.20% respectively. The decreased miR-3175 expression resulted in the abnormal expression of apoptosis related protein p53, Bcl-2, cytochrome C, and Caspase-3 (P<0.05).
Conclusion
The miR-3175 expressed highly in human glioma. The decreased expression level may effectively inhibit the growth and invasion of glioma cells, and promote apoptosis.
Key words:
Glioma; microRNA-3175; Cell proliferation; Apoptosis; InvasionRead More
Publication Year: 2016
Publication Date: 2016-08-28
Language: en
Type: article
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