Title: Overexpression of miR-106a inhibits proliferation and induces apoptosis of glioma cells
Abstract:Objective To investigate the effect of miR-106a on proliferation and apoptosis of glioma cells.Methods MiR-106a levels in glioma tissues of different grades and glioma cell lines(T98G,SHG44,U87,U251,U...Objective To investigate the effect of miR-106a on proliferation and apoptosis of glioma cells.Methods MiR-106a levels in glioma tissues of different grades and glioma cell lines(T98G,SHG44,U87,U251,U373) were measured with quantitative real-time polymerase chain reaction(qRT-PCR).miRNA oligonucleotides were transfected into U87 and U251 using lipofectamine 2000 reagen at the time of 70%~90% confluence.The in vitro growth ability of transfected cells was determined by dimethyl thiazolyl diphenyl tetrazolium(MTT) assay,and the cell-cycle distribution was analyzed using flow cytometry(FCM).After MTT and cell-cycle distribution analysis,the effect of miR-106a on apoptosis was tested by annexin V and propidium iodide(PI) double staining.Results The expression of miR-106a in both glioma specimens and cell lines was significantly lower than that in NATs.Moreover,miR-106a expression was lower in grade III and grade IV glioma compared to grade I and grade II tumors.After miR-106a mimic,inhibitor or scrambled control was transfected in human glioma cells.The results showed that overexpression of miR-106a could suppress the glioma cells proliferation and increase the apoptosis.Conclusion miR-106a induces apoptosis and inhibits proliferation of glioma cells and it may play a potential role in glioma therapy.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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