Title: Expression of microRNA-34b in glioma tissues and its effects on proliferation, apoptosis and invasion of the human glioma cell line A172
Abstract: Objective
To investigate the expressions of microRNA (miRNA, miR)-34b in glioma tissues and its effects on proliferation, apoptosis and invasion of the human glioma cell line A172.
Methods
Seventy-three patents with glioma undergoing elective surgery in the department of Neurosurgery in the Fifth Affiliated Hospital of Zhengzhou University from February 2012 to August 2015 were selected. In the same period, 30 cases of normal brain tissues from patients with traumatic injury undergoing intracranial decompression were selected as the control group. The human glioma cell line A172 cells were cultured. According to the differences of transfections, the cells were divided into miR-46b transfected group, negative control group and blank control group. The expression levels of miR-34b in glioma and the control group tissues and different transfected groups were detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) technique. The cell proliferations in different transfected groups were tested using methyl thiazol tetrazolium (MTT) assay. The apoptosis rate of different transfected groups was measured using flow cytometry. The cell migration and invasion abilities were tested using Transwell experiment.
Results
The relative expression levels of miR-34b in glioma tissues were 1.26±0.11, significantly lower than 1.73±0.14 in the control group with the difference being statistically significant (t=19.527, P<0.01). The relative expression levels of miR-34b in glioma tissues were related with World Health Organization (WHO) grade and Karnofsky (KPS) scores (P<0.05). The relative expression levels of miR-34b in miR-46b transfected group were 0.91±0.11, statistically significantly higher than in the negative control group and blank control groups 0.62±0.09 and 0.64±0.10 respectively (F=147.399, P<0.01). As compared with the negative control group and blank control group, the cell proliferations in miR-46b transfected group were significantly inhibited (P<0.05). The apoptosis rate in miR-46b transfected group was (22.4±5.1)%, which was significantly higher than in the negative control group and blank control groups [(10.6±3.2)% and (10.1±3.6)%, respectively] (F=131.894, P<0.01). The numbers of cell migration and invasion in miR-34b transfected group were less than in the negative control group and blank control group with the difference being statistically significant (P<0.05).
Conclusion
MiR-34b were lowly expressed in glima tissues. Up-regulation expression of miR-34b could accelerate the apoptosis of glioma cells and inhibit the proliferation, migration and invasion of glioma cells..
Key words:
MicroRNA-34b; Glioma; Cell proliferation; Cell invasion
Publication Year: 2016
Publication Date: 2016-12-08
Language: en
Type: article
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