Title: Glucose pretreatment relieves high glucose-induced myocardial apoptosis in vitro
Abstract: Objective To explore the effect of glucose pretreatment on neonatal rat cardiomyocytes (NRCs) exposed to high glucose. Methods The experiment was divided into control group, high glucose (HG) group and glucose pretreatment (PG) group. DMEM+5mmol/L D-glucose cultured for 6h as the control group. To mimic a type 2 diabetic mellitus animal model, the cells were exposed to D-glucose at a final concentration of 50mmol/L for 96h (HG group), and pretreated with glucose (10mmol/L) for 4h (PG group) before exposure to high glucose, repeated 3 times and 4h DMEM (5mmol/L D-glucose) was the time interval. MTS was performed to detect myocardial cell activity. Cardiomyocyte apoptosis was analyzed by TUNEL assay. Mitochondrial membrane potential was detected with JC-1 staining. The expression levels of apoptosis related proteins Bcl-2, Bax and caspase-3 were detected by Western blotting. Results Compared to the control group, cell activity in HG group declined significantly in MTS assay (P 0.05). Conclusion Glucose-pretreatment may increase the cardiomyocytes activity, reduce apoptosis, and extenuate mitochondrial injury, so relieve the high glucose-induced toxicity on NRCs.
DOI: 10.11855/j.issn.0577-7402.2018.09.05
Publication Year: 2018
Publication Date: 2018-09-01
Language: en
Type: article
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