Title: The lipoic acid influenced apoptosis rate of the podocyte induced by high glucose by endoplasmic reticulum stress
Abstract: Objective
To cultivate mice podocyte with high glucose and lipoic acid, to observe the apoptosis rate and the change of endoplasmic reticulum stress (ERS) marker : GRP78 and Caspase-12. To investigate the relationship between endoplasmic reticulum stress and podocyte apoptosis.
Methods
Already differentiated mice podocyte were divided into 4 subgroups: normal glucose concentration group, (5.6 mmol/L), mannitol hypertonic control group(25 mmol/L), high glucose group(25 mmol/L), high glucose + lipoic acid group (25 mmol/L+ 500 μmol/L). Index and detection method: after 48 h, to detect cell apoptosis rate by flow cytometer, to detect the expression of GRP78 and Caspase-12 mRNA with reverse transcription-polymerase chain reaction (RT-PCR) technology, to detect the GRP78 and Caspase-12 protein expression with Western blotting technology. The apoptosis rate and expression of GRP78 and Caspase-12 mRNA and protein between the groups were compared by LSD-t test.
Results
About cell apoptosis rate: (1)The apoptosis rate between normal glucose concentration group and hypertonic group were not statistically significant((3.4±0.6)% vs (3.7±0.9)%, t=0.52, P>0.05); The apoptosis rate of high glucose group was significantly higher than normal glucose concentration group((14.0±1.1)% vs (3.4±0.6)%, t=9.12, P<0.05); The apoptosis rate of lipoic acid + high glucose group was significantly decreased than high glucose group((14.0±1.1)% vs (7.8±0.6)%, t=7.73, P<0.05). (2)The expression of GRP78 and Caspase-12 mRNA in high glucose group increased significantly than normal glucose concentration group (2.62±0.57 vs 0.85±0.24, 10.04±2.28 vs 1.28±0.97; t=6.75, 25.84, all P<0.05); The expression of GRP78 and Caspase-12 mRNA in lipoic acid + high glucose group, decreased significantly than high glucose group(0.82±0.22 vs 2.62±0.57, 4.98±0.55 vs 10.04±2.28, t=3.83, 11.83, all P<0.05). (3)The expression of GRP78 and Caspase-12 protein in high glucose group increased significantly than normal glucose concentration group(2.48±0.19 vs 0.37±0.04, 2.07±0.18 vs 0.47±0.08, t=17.55, 12.34, all P<0.05); The expression of GRP78 and Caspase-12 protein in lipoic acid + high glucose group decreased significantly than high glucose group(0.61±0.15 vs 2.48±0.19, 0.89±0.18 vs 2.07±0.18, t=4.42, 3.25, all P<0.05).
Conclusion
(1)High glucose can improve apoptosis of podocyte by the endoplasmic reticulum stress reaction. (2) The lipoic acid can reduce the endoplasmic reticulum stress reaction to protect the podocyte.
Key words:
Lipoic acid; High glucose; Kidney podocytes; Endoplasmic reticulum stress thioctic acid
Publication Year: 2014
Publication Date: 2014-07-27
Language: en
Type: article
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