Title: Effects of glutathione pretreatment on glucose metabolism after hepatic ischemia/reperfusion
Abstract: Objective To observe the effects of glutathione pretreatment on glucose metabolism after hepatic ischemia/reperfusion.Methods Seventy-eight SD rats were randomly divided into ischemia/reperfusion group(group IR),glutathione group(group G),and control group(group C),with 26 rats in each group.The HIRI model was established in groups IR and G by ischemia for 40 min and reperfusion for 2 h or 6 h.The concentrations of blood glucose(BG) and plasma insulin,the activity of superoxide dismutase(SOD),the levels of malondialdehyde(MDA)were determined at 2 h or 6 h after reperfusion in 8 rats of each group.Hyperglycemic clamp test was performed in another 5 rats of each group,so that to determine the concentration of insulin and to calculate the glucose metabolism rate(GMR) and the insulin sensitivity index(ISI).Results Compared with those in group C,the concentrations of BG and the levels of MDA were significantly higher in group IR after ischemia-reperfusion,but the activity of SOD and the concentrations of plasma insulin were remarkably lower(P0.05).The hyperglycemic clamp test showed that the insulin secretion level,the glucose metabolism rate and the insulin sensitivity index in group IR were obviously lower than those in group C.Compared with those in group IR,the levels of insulin secretion,the GMR and the ISI were higher in group G.Conclusion Glutathione pretreatment has a protection against the disturbance of glucose metabolism during the hepatic ischemia/reperfusion injury(HIRI).The mechanism of protection may be associated with its scavenging of oxygen free radical and inhibition of lipid peroxidation.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot