Title: Determination of baicalin in Shuanghuangbaidu Granules by RP-HPLC
Abstract:A RP-HPLC method has been developed to determine the baicalin in Shuanghuangbaidu granules,using C_(18) column with a mobile phase of methanol-water-phosphoric﹙45∶55∶0.2﹚at a flow rate of 1mL/min and ...A RP-HPLC method has been developed to determine the baicalin in Shuanghuangbaidu granules,using C_(18) column with a mobile phase of methanol-water-phosphoric﹙45∶55∶0.2﹚at a flow rate of 1mL/min and a DAD detector at 280 nm.The baicalin of the samples were extracted with 70% alcohol by ultrasonic and determined by the RP-HPLC method.The linear range of baicalin was 0.16~0.96 μg(r=0.9997,n=6).The average recovery was 102.2% with RSD of 1.57%(n=9).Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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