Title: Determination of baicalin in Baikejing granules(for children)by HPLC
Abstract:Objective To establish a HPLC method for determination of baicalin in Baikejing granules.Methods HPLC was performed on Gimini ODS(4.6 mm×250 mm,5 μm)column with acetonitrile-0.03 mol/L ammonium acetat...Objective To establish a HPLC method for determination of baicalin in Baikejing granules.Methods HPLC was performed on Gimini ODS(4.6 mm×250 mm,5 μm)column with acetonitrile-0.03 mol/L ammonium acetate(containing 0.25% formic acid)(80∶20)as mobile phase at 40 ℃column temperature.Its flow rate was 1.0 mL/min.The detecting wavelength was 316 nm and the injection volume was 20 μL.Results The Baicalin was linear in the concentration range of 1.06-10.6 μg/mL(r =0.999 5),and the average recovery was 100.5%(RSD =0.67%,n=9).Conclusion This method is simple,accurate,reproducible and can be used for quality control of this preparation.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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