Title: The Content Determination of Baicalin in Ganmaozhike granules by HPLC
Abstract:Objective To establish a HPLC method for the assay of baicalin in Ganmaozhike granules.Methods The analytical column was Diamonsil C18(250 mm×4.6 mm,5 μm).The mobile phase was consisted of CH3OH-H2O-H...Objective To establish a HPLC method for the assay of baicalin in Ganmaozhike granules.Methods The analytical column was Diamonsil C18(250 mm×4.6 mm,5 μm).The mobile phase was consisted of CH3OH-H2O-H3PO4(65∶35∶0.3).The flow rate was 1.0 mL/min and column temperature was room temperature.The detective wavelength was 278 nm.Results The baicalin linenarity renge was 32.3~323.8 μg/mL.The average recovery was 100.5%,and the RSD was 1.2%.Conclusion This method was simple and accurate,which could be used for the quality control of Ganmaozhike granules.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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