Title: Determination of baicalin in Ganmaozhike granules by HPLC
Abstract:Objective To establish an HPLC method for content determination of Ganmaozhike granules.Methods The separation was performed with an UltimateTM XB-C18 column(4.6 mm × 200 mm,5 μm)and the mobile phase ...Objective To establish an HPLC method for content determination of Ganmaozhike granules.Methods The separation was performed with an UltimateTM XB-C18 column(4.6 mm × 200 mm,5 μm)and the mobile phase was methol-0.4%phosphoric acid(50:50).The flow rate was 1.0 ml/min and the wavelength of detection was 277 nm.The column temperature was 40℃.Results The liner range of baicalin was 0.058~1.16 μg(R2=1.000)and the average recovery was 99.54%,RSD=0.85%(n=6).Conclusion This method is accurate、simple and can be used for the quality control of Ganmaozhike granules.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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