Title: Determination of Content of Baicalin in Wenyixiao Granules by HPLC
Abstract:The content of baicalin in Wenyixiao granules was determined by HPLC.The Symmetry C18 (3.9mm×150,5μm) was adopted as chro- matagraphic column,the mobile phase was acetonitrile-0.2% phosphoric acid sol...The content of baicalin in Wenyixiao granules was determined by HPLC.The Symmetry C18 (3.9mm×150,5μm) was adopted as chro- matagraphic column,the mobile phase was acetonitrile-0.2% phosphoric acid solution (45:55),the wavelength was 280nm,the velocity of flow was 1.0ml/min and the column temperature was 25℃.The result showed that baicalin had excellent linear relationship between 0.0248~0.124mg/mL (r= 0.9993).The average recovery was 96.93% (RSD=0.92%) and we got the content of baicalin in the Wenyixiao granules was 9.0 mg/g.This method was brief,exclusive,and accurate and had good reproducibility.It could be used to control the quality of Wenyixiao granules.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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