Title: Determination of Baicalin in DanShiTong Capsules by HPLC
Abstract: Objective To establish a method for the content determination of baicalin in DanShiTong Capsules,so as to effectively control its quality.Methods The content of baicalin was detected by HPLC.The operating conditions were Promosil C18(4.6×250 mm,5μm) column,The mobile phase consisted of methanol-0.3% phosphoric acid(20∶80).The detection wavelength was at 276 nm,the flow rate was 1.0mL/min,the column temperature was 25℃,external standard method was employed.Results The linear ranges was obtained between 0.054~1.08μg(r=0.9997,n=5) of baicalin.The average recovery rate was 98.2% respectively with corresponding RSD of 1.1 %(n=9).Conclusion The method is simple,convenient,rapid and reliable with good reproducibilty,Which can be used for the content determination of baicalin in DanShiTong Capsules
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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