Title: Effect of burn injury on the expression of cytokine genes in skin
Abstract:Objective To study the effects of burn injury on the expression of cytokine genes.Methods Male ICR mice, 20~25 g, were anesthetized with intraperitoneal pentobarbital, which resulted in 20% Ⅲ degree b...Objective To study the effects of burn injury on the expression of cytokine genes.Methods Male ICR mice, 20~25 g, were anesthetized with intraperitoneal pentobarbital, which resulted in 20% Ⅲ degree burn injury. The unburned skin and serum were collected at 24 hours after the burn injury. Total RNAs were isolated from the skin. The expression of TNF-α, IL-1α and IL-6 genes were estimated by reversal transcription -polymerase chain reaction (RT-PCR). The level of TNF-α and IL-6 were also measured by ELISA.Results 20% Ⅲdegree burn injury could induce the expression of TNF-α and IL-6 genes in the unburned skin whereas the burn injury had no effects on the expression of IL-1α. The expression of TNF-α and IL-6 genes could not be detected in the skin of control animals. The burn injury increased the level of TNF-α in skin, but not in serum.The level of TNF-α in skin and serum was much lower in the control. Furthermore, the concentration of IL-6 was elevated both in the skin and in the serum after the burn. On the contrary, the level of IL-6 both in the skin and in the serum was low in the control group. There were significant differences between two groups (P0.01).Conclusion Burn injury might activate the skin immune system, and make the skin function as a cytokine-generating organ.Read More
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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Cited By Count: 1
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