Title: Inhibition of activin receptor-like kinase 5 affects acute lung injury of severely burnt rats
Abstract:Objective To investigate the potential roles of Smads signal transduction pathway in earlier period of pulmonary fibrosis formation by investigating the expression of proinflammatory cytokines and ext...Objective To investigate the potential roles of Smads signal transduction pathway in earlier period of pulmonary fibrosis formation by investigating the expression of proinflammatory cytokines and extent of inflammatory lung injury.Methods Twenty-four SD rats were randomly divided into group of control,burn,SB431542 early treatment and SB431542 later treatment with 6 rats for each.Rats other than in the control group were burnt at 98℃ for 15 s with a constant burning apparatus to prepare for the 3rd degree burns of 30% of total body surface area(TBSA),and rats of the control group were burnt in water at 37℃ for 15s.Rats of the control group received no fluid resuscitation,while rats of the other groups were abdominally administrated with lactated Ringer′s solution at 40 ml/kg body weight for fluid resuscitation respectively after the burn.Rats of early treatment group were treated with SB431542 immediately,at 1×24 h and 2×24 h after the burn respectively.Rats of later treatment group were treated with SB431542 at 3×24 h,4×24 h and 5×24 h after the burn respectively.The lungs of all rats were obtained at 7 d after the burn.The mRNA expression of TNF-α and IL-1β in the tissues of lung were tested by real time PCR.Lung specimens were also double blindly examined and evaluated by Szapiel′s pathological scores.Results Early inhibition of activin receptor-like kinase 5(ALK5) increased production of TNF-α and IL-1β mRNA.Pathological changes suggested that inflammatory response could be increased by the early inhibition of ALK5 but not the late one.Conclusions Smads signal transduction pathway involved in the inflammatory response of lung tissue after burn.In the acute phase response of inflammatory lung injury,early inhibition of ALK5 could increase mRNA production of TNF-α and IL-1β,which further increased the expression of proinflammatory cytokines,and aggravated burn-induced inflammatory lung injury,while late inhibition of which did not show the effects.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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