Title: Expressions of P56,IFN-α and IFN-β mRNAs in major organs of severe burn mice and effects of KPV and anaesthesia
Abstract:Objective To investigate the expressions of P56, IFN-β, IFN-α mRNAs and the effects of KPV, a COOH-terminal tripeptide sequence of alpha-melanocyte stimulating hormone [alpha-MSH(11-13)], and anaesthe...Objective To investigate the expressions of P56, IFN-β, IFN-α mRNAs and the effects of KPV, a COOH-terminal tripeptide sequence of alpha-melanocyte stimulating hormone [alpha-MSH(11-13)], and anaesthesia on the expressions in severe burn injured mice.Methods Eighty mice were randomly and equally divided into 4 groups: control, burn (TBS15%Ⅱ), burn+KPV (injection of 40 μg per animal immediately after burn), and burn+anaesthesia (injection of 0.4 mg/kg diazepam and 10 mg/kg ketamine immediately after burn). All animals were sacrificed in 3 h after burn respectively. The expressions of P56, IFN-β, and IFN-α mRNAs in liver, spleen, lung and kidney tissues were detected with RT-PCR. Results P56 was expressed highly in liver, spleen, lung, and kidney in normal control group, and was obviously increased in kidney in burn group and burn+KPV group, but that in burn+anaesthesia group had no significant difference with that in normal control. IFN-β was not expressed in live and lightly expressed in spleen, lung and kidney, and its expression in lung was significantly increased in burn group and burn+anaesthesia group, but mildly decreased in burn+KPV group. IFN-α mRNA was expressed in all detected organs in normal control, and highly expressed in kidney of burn group and burn+KPV group, but not in burn+anaesthesia group. Conclusion These results indicate that the increased IFN expressing induce the up-regulation of P56, suggesting which may be involved in the disorder of early post trauma stress.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot