Title: Determination the Content of Rutin in the Zhiyanxiao Keli by HPLC
Abstract:Objective:An HPLC method was established for the determination of Rutin.Methods:Using Merck Lichrospher C_(18)(5 μm,250 mm×4.6 mm) with the mobile phase consisiting of methanol-1% glacial acetic acid(...Objective:An HPLC method was established for the determination of Rutin.Methods:Using Merck Lichrospher C_(18)(5 μm,250 mm×4.6 mm) with the mobile phase consisiting of methanol-1% glacial acetic acid(32∶68);The detective wavelength was 257 nm;The flow rate was 1 mL·min~(-1).Results:The standard curve was liner with the range of 0.27~7.11 μg(n=6,RSD=0.91%),r=(0.999 5). The average recovery was 99.54%,RSD 0.91%.Conclusion:The method is simple and rapid which can be used for the quality control of Zhiyanxiao Keli.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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