Title: Determination of Baicalin,Chlorogenic Acid and Rutin in the Yin Huang Buccal Tablets by HPLC with Gradient Elution
Abstract:A method for simultaneous determination of baicalin,chlorogenic acid and rutin in the yin huang buccal tablets was developed.A hypersil ODS C18(4.6mm×200mm,5μm) column was used.A mixture of methanol-w...A method for simultaneous determination of baicalin,chlorogenic acid and rutin in the yin huang buccal tablets was developed.A hypersil ODS C18(4.6mm×200mm,5μm) column was used.A mixture of methanol-water-acetic acid(10:88:2)was used as mobile phase A and the mixture of methanol-water-acetic acid(88:10:2) as mobile phase B.Phase A-phase B in gradient elution:0 min (97:3)-10min(73:27)-25min(20:80)-36min(20:80) at a flow rate of 1.0mL/min.The detection wavelength was set at 327 nm and 280 nm and the column temperature was 25℃.The calibration curve was linear in the range of 0.051—0.512μg/mL for baicalin(r=0.9990),average recovery was 98.23% and RSD was 2.13% (n=6).The calibration curve was linear in a range of 0.059—0.592μg/mL for chlorogenic acid(r=0.9996),average recovery was 97.61% and RSD was 2.13%(n=6).The calibration curve was linear in a range of 0.087—0.868μg/mL for rutin(r=0.9992),average recovery was 98.56% and RSD was 1.13%(n=6).The method is sensitive,reproducible,and suitable for the quality control of the yin huang buccal tablets.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot