Title: Determination of hesperidin in Jingouyin by HPLC
Abstract:OBJECTIVE To establish an HPLC method for determination of hesperidin in Jinguoyin.METHODS A Agilent zoxbax C_ 18 (15cm×4.6mm,3.5μm) was used with a mobile phase of acetonitrile-0.2%phosphoric acid so...OBJECTIVE To establish an HPLC method for determination of hesperidin in Jinguoyin.METHODS A Agilent zoxbax C_ 18 (15cm×4.6mm,3.5μm) was used with a mobile phase of acetonitrile-0.2%phosphoric acid solution(20∶80),the flow rate was 1.0mL/min,the detection wavelength was at 284nm.RESULTS The calibration curve was linear within the range of 0.35~ 7.0 μg/mL(r=0.999 2),and the average recovery of hesperidin was 99.21%(RSD=1.12%,n=6).CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of Jinguoyin.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot