Title: Simultaneous determination of paeoniflorin,emodin and tanshinoneIIA in Yigankang Keli by HPLC
Abstract:Objective To establish an HPLC method for the simultaneous determination of paeoniflorin,emodin and tanshinoneⅡA in Yigankang Keli.Methods The column was Thermo C18(250 mm ×4.6 mm,5 μm) with the mobil...Objective To establish an HPLC method for the simultaneous determination of paeoniflorin,emodin and tanshinoneⅡA in Yigankang Keli.Methods The column was Thermo C18(250 mm ×4.6 mm,5 μm) with the mobile phase composed of methanol-0.1%phosphoric(30∶70).The flow rate was 1.0 mL·min-1,the detection wavelength was 230 nm,the column temperature was 35 ℃ and the injection volume was 10 μL.Results The linear ranges of paeoniflorin,emodin and tanshinoneⅡA were 0.010 45-0.125 4(r=0.999 3,n=6),0.010 21-0.122 5 μg(r=0.999 0,n=6),and 0.010 56-0.126 7 μg(r=0.999 2,n=6).The average recoveries of paeoniflorin,emodin and tanshinoneⅡA were 99.0%,99.3% and 99.1%.RSD was 1.2%,1.1% and 1.1%(n=9).Conclusion The method is simple,accurate,sensitive and repeatable,which can be used to control the quality of Yigankang Keli.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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