Title: Determination of Chlorogenic Acid in Reduping Keli by HPLC
Abstract:Objective To establish an HPLC method for the determination of Chlorogenic acid in Reduping Keli. Methods The determination was performed by HPLC using an YMC ODS-A column (150 mm×4.6 mm, 5 μm), with ...Objective To establish an HPLC method for the determination of Chlorogenic acid in Reduping Keli. Methods The determination was performed by HPLC using an YMC ODS-A column (150 mm×4.6 mm, 5 μm), with mobile phase consisted of acetonitrile-0.2%H_3PO_4 solution (10 : 90) at a flow rate of 1.0 mL/min and a detection wavelength of 327 nm. Results The calibration curve for Chlorogenic acid was linear in the concentration range of 0.0085~0.084 8 mg/mL (r=0.9999). The average recovery was 102.60 % (RSD=1.69%, n =6). Conclusion The method is simple, rapid and applicable for the quality control of Reduping Keli.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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