Title: Study on Anther Culture of Strawberry Lasong No.7
Abstract: [Objective] The aim of research was to screen the optimal medium of anther culture of srawberry Lasong No.7.[Method] The relationship between bud size and anther development was builded.With the anthers of single nuclear aside period as explants,with MS as basic medium,added different hormones and cytokinin to establish regeneration system,the effects of different hormone combinations on anther culture of strawberry were studied.[Result] Optimal sterilizing method was treating with 70% alcohol 30 s;the optimal pretreatment method was low temperature at 4 ℃ for 24 hour.The best medium for callus induction was MS supplemented with 0.2 mg/L NAA and 2.0 mg/L 6-BA;the best medium for shoot differentiation was MS supplemented with 2.0 mg/L 6-BA and 0.1 mg/L KT,the value-added multiplier was 3-4;the best rooting medium was 1/2 MS,the rate of rooting was 100%.[Conclusion] The excellent regeneration system of strawberry anther was obtained.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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