Title: Study on Embryo Regeneration System of Onion
Abstract: Taking the onion embryo as explants,the tillered-onion regeneration system was established with MS as the basic culture induction,the effect factors,such as different onion varieties and different combination of hormone treatment on the regeneration process was analiped.The results showed that‘Fenhuang Jingiu'onion varieties was suitable for culture induction with the highest callus induction rate;MS+4mg/L 2-4,D+1mg/L 6-BA was the most suitable for callus induction,the rate of callus induction reached 72.1%.Different varieties had the highest rate of callus with 72.1%;addition of 1.0mg/L TDZ and 0.5mg/L NAA to the MS medium promoted a the highest frequency of regeneration(78.3%);the medium which contained 1/2MS+0.01mg/L NAA induced white strong roots,rooting rate reached100%;the regenerated plantlets were domesticated and transplanted in greenhouse;the survival rate was 95%.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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