Title: Anther culture and plant regeneration of Robinia hispida
Abstract: The anthers of Robinia hispida were cultured and the results showed that the highest induction rate of callus was 41. 5% when they were cultured on MS medium supplemented with 2,4-D 0. 1 mg/L and BA 3. 0 mg/L for 20 days. Green buds formed from the callus after 2 months subcultured on MS medium supplemented with BA 5. 0 mg/L. When the shoots grew to 2-3 cm long,they were detached and trans-ferred to MS medium supplemented with IBA 1. 0 mg/L for rooting. The shoots produced roots within 2 weeks of culture and formed complete plantlets. At the same time,the effects of pretreatment of low tem-perature at 4 C and the concentration of sucrose on anther culture were studied and discussed.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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