Title: Study on the Tissue Culture and Plant Regeneration of Good Quality Sweet Potato Variety Nanshu 88
Abstract: [Objective] The purpose of the study was to lay foundation for in vitro screening and genetic transformation of Nanshu 88.[Method] The leaf and leafstalk of Nanshu 88,as explants,were put into the media with different hormone combinations for tissue culture and plant regeneration so as to study the influences of different hormones and explants on the plant regeneration of Nanshu 88.[Result] The effect of solely using 6-BA and NAA to induce callus was not perfect.2,4-D with too high concentration was not favorable for the root formation and bud inducement of explants.On the media of MS+NAA 1 mg/L+6-BA 0.5 mg/L and MS+2,4-D 0.05 mg/L+KT 1 mg/L,the callus induction and root differentiation of the 2 explants all achieved 100 %,the bud differentiation rates of leaf were 20 % and 7 % resp.,the bud differentiation rates of leafstalk were all 7 % and the regenerated plants were obtained successfully.On the same media,the growth conditions of calli from different explants had little difference and the differentiations of organs showed some difference.[Conclusion] The more suitable media for tissue culture of Nanshu 88 were MS+NAA 1 mg/L+6-BA 0.5 mg/L and MS+2,4-D 0.05 mg/L+KT 1 mg/L.The differentiation ability of Nanshu 88 leaf was bigger than that of leafstalk.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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