Title: Inhibition of hydroxysafflor yellow A against rat cardiomyocyte apoptosis
Abstract:Objective To investigate the inhibitory effect of hydroxysafflor yellow A(HSYA) against cardiomyocyte apoptosis.Methods Rat myocardial apoptosis was induced by ip isoproterenol(ISO) and then HSYA was ...Objective To investigate the inhibitory effect of hydroxysafflor yellow A(HSYA) against cardiomyocyte apoptosis.Methods Rat myocardial apoptosis was induced by ip isoproterenol(ISO) and then HSYA was ip given to alleviate the apoptosis.Rat neonatal cardiomyocyte apoptosis was triggered by oxygen/glucose-deprivation/reperfusion(OGDR) injury.Cardiac tissue was observed by transmission electron microscopy(TEM) and cardiac cell apoptosis was assayed by terminal deoxynucleotidyl transferase dUTP nick-end labeling(TUNEL) staining.Bcl-2 and bax gene expression was observed by immunohistochemical staining and RT-PCR.The effect of HSYA to inhibit cardio myocyte apoptosis was assayed with the Flow Cyto Meter.Rhodamine 123 staining was used to measure the change of mitochondrial membrane potential.Results TEM showed that mitochondrion swelling and nucleus pyknosis were alleviated by HSYA treatment.It was shown by TUNEL staining that 60,120,or 240 mg/kg HSYA decreased the apotosis rate of cardiomyocyte in rats with myocardial ischemia.In 120 mg/kg HSYA-treated rats,Bax expression was downregulated compared with NS treated rats.In cultured OGDR cardiomyocytes,HSYA inhibited the decrease in mitochondrial membrane potential.Conclusion The mechanisms involved in the cardioprotective effect of HSYA are inhibition of cardiomyocyte apoptosis.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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