Title: Shenmai injection protects rat cardiomyocytes from angiotensin II-induced apoptosis in vitro
Abstract:AIM: To study the protective effects of Shenmai injection, a Chinese medicine, on angiotensin Ⅱ (AngⅡ)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism. METHODS: Cultured cardio...AIM: To study the protective effects of Shenmai injection, a Chinese medicine, on angiotensin Ⅱ (AngⅡ)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism. METHODS: Cultured cardiomyocytes from neonatal rats were stimulated with AngⅡ. Cell viability were measured by MTT, and apoptosis was evaluated suing Hoethst33258 fluorescent dye staining and flow cytometry. Fluo-3/AM was used to test the change in intracellular free calcium. RESULTS: It was found that incubation with AngⅡ (10 -7 mol/L) for 48 h increased cardiomyocyte apoptosis, Shenmai injection (0 5 g/L, 1 0 g/L) inceased myocyte viability ( P0 05 ). Shenmai injection (1 0 g/L) significantly decreased the AngⅡ-induced rat cardiomyocyte apoptosis ( P0 05 ) and decreased fulorescent intensity of intracellular calcium. CONCLUSION: Shenmai injection has a significant inhibitory effect on AngⅡ-induced rat cardiomycoyte apoptosis in vitro by alleviating intracellular calcium overload. [Read More
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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Cited By Count: 1
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