Title: Protective effects of hydroxysafflor yellow A against MPP~+-induced apoptosis in SH-SY5Y cells
Abstract:Objective To investigate the neuroprotective effect of hydroxysafflor yellow A(HSYA)on MPP+-induced apoptosis in SH-SY5 Y cells and its related mechanisms.Methods Cell viability was analyzed using MTT...Objective To investigate the neuroprotective effect of hydroxysafflor yellow A(HSYA)on MPP+-induced apoptosis in SH-SY5 Y cells and its related mechanisms.Methods Cell viability was analyzed using MTT assay.Cell apoptosis was measured by flow cytometry.The level of reactive oxygen species(ROS)within the cells was analyzed by fluorometric probe DCFH-DA assay and mitochondrial transmembrane potential was revealed by rhodamine123 staining.The expressions of cytosolic Cyt-C,Bcl-2,Bax,Caspase-3 and Caspase-9 were analyzed by Western blot.Results The cell viability was significantly decreased by 1 mmol/L MPP+,and the percentage of apoptosis increased to(38.6±1.8)%,but was reversed by HSYA pretreatment of different concentrations(15,60 and 120μmol/L)(P0.001,P0.01,respectively).Pretreatment of HSYA reversed the increase of ROS,cytosolic Cyt-C,Caspase-3 and Caspase-9(P0.001,P0.05,P0.05,P0.05,respectively),decrease of mitochondrial transmembrane potential and Bcl-2/Bax ratio induced by MPP+in SH-SY5 Y cells(P0.01,P0.01,respectively).Conclusion HSYA pretreatment can increase cell viability to prevent MPP+-induced cell apoptosis.The mechanism may be correlated to the inhibition of mitochondrial apoptotic pathway.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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