Title: A Comparative Analysis on Staining Eff Iciencies of Four Fluorescent Dyes in Preparative Agarose Gel Electrophoresis
Abstract:Objective To investigate the staining effect of Genefinder,Geneview and SYBER greenI to the DNA/RNA samples in the gels and evaluate the possible application of them in routine nucleic acid staining a...Objective To investigate the staining effect of Genefinder,Geneview and SYBER greenI to the DNA/RNA samples in the gels and evaluate the possible application of them in routine nucleic acid staining as a substitute for Ethidium Bromide(EB).MethodsComparative analysis was performed on the RNA/DNA staining efficiencies of four fluorescent dyes in preparative agarose gel electrophoresis.Results There was no obvious difference among staining efficiencies of four fluorescent dyes(P0.05).Conclusion The new nucleic stains of Genefinder,Geneview and SYBER greenI may substitute for EB as general dyestuff to stain RNA/DNA in the gel.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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